NEUROG1 and colorectal cancer: We obtained 904 colorectal cancer specimens and quantified DNA methylation in the 16 loci [CACNA1G, IGF2, NEUROG1, RUNX3, SOCS1, CDKN2A (p16), CRABP1, MLH1, CHFR, HIC1, IGFBP3, MGMT, MINT1, MINT31, p14 (CDKN2A/ARF), and WRN] by real-time PCR (MethyLight [30]) assays.