In this study, we made use of HA-collagen composite scaffold, NSCs and NT-3 as a nerve guide, effecter cells and neurotrophic/neuroprotective factor, respectively, and implanted the conduit of NSCs-implanted NT-3-supplemetned HA-collagen composite scaffold onto rabbits having induced peripheral nerve gap defect and evaluated the therapeutic effects on peripheral nerve lesion. Here, NTF3 is linked to peripheral nerve lesion.