To further confirm the promoter CGI hypermethylation-mediated FBLN1 silencing in primary gastric carcinomas, FBLN1 expression and FBLN1 promoter methylation in primary gastric carcinoma tissues, adjacent non-tumour gastric tissues and normal gastric tissues were analysed by quantitative real-time RT–PCR and MSP, respectively. The gene discussed is FBLN1; the disease is neoplasm.