To confirm that reactivation of LRRC4 mRNA expression in glioma cell lines was caused by demethylation of the LRRC4 promoter, methylation-specific PCR was used to detect methylation status changes in the LRRC4 promoter in the SF767 and SF126 cell lines after 5-Aza-dC treatment. Here, LRRC4 is linked to central nervous system cancer.