Confluent monolayers of MCF7 cells (well-differentiated human breast cancer cells) were tested for the presence of transiently-transfected human Flag-TRAF4 at AJs (Figure 4 A), desmosomes (Figure 4 B) and TJs (Figure 4 C), as identified by their respective markers E-cadherin, desmoplakin and occludin using appropriate antibodies and fluorescence confocal microscopy. The gene discussed is OCLN; the disease is breast cancer.