In order to model Barrett's Esophagus, EPC2-hTERT Myc cells alone or with Cdx1 were cultured in a 3-dimentional culture system (organotypic culture) previously utilized in our lab to mimic the microenvironment of the esophagus to study esophageal tumorigenesis [34]. This evidence concerns the gene CDX1 and esophageal adenocarcinoma.