Copy number changes relative to the Line-1 gene and the calibrator were determined by the formula (TEGFR/TLine-1)/(CEGFR/CLine-1), where TEGFR/TLine-1 are quantity from tumor DNA by using EGFR and Line-1, and CEGFR/CLine-1 are quantity from calibrator by using EGFR and Line-1 [19,20]. This evidence concerns the gene EGFR and neoplasm.