Strikingly, expression of one of the primary Notch target genes, Hes1, does not correlate with the abundance of the Val1744-NICD fragment, suggesting that Notch pathway activity may only fully drive Hes1 expression in some CRC lines and that other pathways could contribute to Hes1 expression regulation in certain CRC cells (Figure 2, second panel from top). Here, HES1 is linked to colorectal carcinoma.