Determination of the MFI showed that the LPS-stimulated peritoneal macrophages expressed lower levels of IL-1β (MFI: 85.28, (IgG: 49.23)) than did ischemia-activated macrophages (MFI: 104.03 (IgG: 46.06)) whereas LPS-stimulated peritoneal macrophages expressed higher levels of TNF-a (MFI: 53.84, (IgG: 14.79)) than did ischemia-activated macrophages (MFI: 39.28 (IgG: 24.11)). The gene discussed is TNF; the disease is ischemia.