IFNG and melanoma: HLA A2 PBMC alone from healthy subjects did not show response to melanoma peptides; a dose dependent IFNγ response, fold increase (IFNγ relevant peptide/CD8)/(IFNγ HIV/CD8), was only seen in PBMC with spiked TIL cells stimulated with relevant peptide, TIL1520 stimulated with gp100209 and gp100pool and TIL1235 stimulated with the MART-1 peptide (Figure 4).