To explore the reliability of the expression signature previously identified by cDNA array, RTQ-PCR of ESR1/ERα, FOS, IGFBP4, MET, BCL2, and SNCG gene expression was examined in a larger set of 35 tumor samples (17 control and 18 TF), including the 18 samples used in the initial screen. This evidence concerns the gene IGFBP4 and neoplasm.