To provide reliable quantitative assessments, we spiked cells from a HLA-A0201 restricted CD8+ CTL clone recognizing gp100280–288 melanoma associated epitope in allogenic WB from a HLA-A0201+ healthy donor and we incubated the mixture for 5 hours in the presence of a 10 μg/ml final concentration of specific or control (Melan-A/MART-127–35) peptide. The gene discussed is MLANA; the disease is melanoma.