SGCG and Creutzfeldt Jacob disease: Analysis of frontal cortex brain homogenate by immunoblotting after limited proteinase K digestion demonstrated the presence of type 3 PrPSc (figure 2), which we have previously observed in sporadic and iatrogenic CJD (Collinge et al. 1996; Wadsworth et al. 1999; Hill et al. 2003) and in kuru (Wadsworth et al. 2008a,c).