While non-CF cell lines cease to produce GM-CSF and IL-6 by 6 hr and IL-8 by 24 hr, CF cells continue to accumulate these cytokines for the duration of the experiment (48 hr) [37], suggesting a diminished ability of CF cells to inactivate NF-κB once it is activated. Here, CXCL8 is linked to cystic fibrosis.