IFN-γ ELISPOT responses to peptide pools containing a total of ninety-six epitopes representing HLA A01 (18 epitopes), HLA A02 (20 epitopes), HLA A03 (20 epitopes), HLA A24 (14 epitopes), HLA B07 (11 epitopes) and HLA-B44 (13 epitopes) supertypes were evaluated in 25 chronic Chagas disease subjects without clinical and radiological evidence of heart disease (G0 status) regardless of their HLA type. This evidence concerns the gene IFNG and heart disorder.