For all nine genes the methylation frequency in BE and D-EAC was significantly higher than each of the squamous tissues (S, S-BE and S-EAC) with the exception of CDKN2A (no difference between BE or D-EAC and S or S-BE), RBP1 (BE vs S-BE and D-EAC vs S or S-BE) and SFRP1 (D-EAC vs S-EAC). This evidence concerns the gene SFRP1 and Barrett esophagus.