The main objective of this work was to compare the humoral immune response in three groups of Plasmodium-infected subjects living in malaria endemic areas of Brazil: (a) subjects with non-cerebral complicated malaria, (b) subjects with uncomplicated malaria, and (c) asymptomatic subjects – investigating the distribution of P. falciparum blood stages-specific isotypic Ab IgG, IgG1, IgG2, IgG3, IgG4 IgM, IgE and IgA. This evidence concerns the gene CD40LG and malaria.