Finally, we show that vaccination of the HCC patient with autologous FCs (DCs/auto-HCC) is associated with enhanced immunological responses, as demonstrated by: 1) augmented DCs function; 2) improved production of IFN-γ in both CD4+ and CD8+ T cells and T-cell proliferation; 3) enhanced induction of CEA and/or WT1-specific CTL responses; and 4) augmented CTL activity against autologous HCC cells in vitro assay. This evidence concerns the gene CD4 and hepatocellular carcinoma.