WT1 and hepatocellular carcinoma: In the present study, we first show that coculture of T cells from healthy donors with the fusion cells (FCs) created by allogeneic HCC cells and immature DCs from the donors (DCs/allo-HCC) results in activation of both CD4+ and CD8+ T cells, as demonstrated by high levels of IFN-γ production and lysis of the CEA- and/or WT1-positive targets restricted in HLA-A2 and/or HLA-A24.