To further analyse the role of MUC4 in the aggressiveness of non-signet ring cell type poorly differentiated gastric adenocarcinomas, MUC4 was ectopically expressed in AGS gastric cancer cell line (using an engineered MUC4 cDNA construct, MUC4minigene (Moniaux et al, 2007)). Here, MUC4 is linked to gastric cancer.