In accordance with this result, the addition of purified IL-6 to spleen cells of 4T1 tumour-bearing mice that were immunised with LM-LLO-Mage-b/2nd, completely inhibited the generation of IFNγ-producing cells upon restimulation with BM cells highly expressing Mage-b, while in the absence of purified IL-6, a high number of IFNγ-producing cells was detected (Figure 6B). This evidence concerns the gene IFNG and neoplasm.