EPAS1 and neoplasm: Although in HIF-2α stromal-negative CRCs, BNIP3 methylation occurred in 61% (33 out of 54) and did not influence prognosis, in the HIF-2α-positive tumours, methylation was observed at almost equal frequency, 52% (68 out of 132), but was associated with poorer patient survival (P=0.006; Figure 2G).