To investigate if the association pattern between HLA class II genes and ICC is dependent on the distribution of HPV-16 variants, we evaluated the HPV-16 variability in 107 patients enrolled in a case-control study [19], previously analyzed for HLA-DRB1, DQB1 and DQA1 polymorphisms. The gene discussed is HLA-DRB1; the disease is intrahepatic cholangiocarcinoma.