To determine whether methylation directly mediates OPCML silencing, carcinoma and lymphoma cell lines (MB231, Hep3B, HepG2, SNU398, SW480 and L1236) were treated with the DNA methyltransferase inhibitor 5-aza-2′-deoxycytidine (Aza), together with or without histone deacetylase inhibitor Trichostatin A (TSA). The gene discussed is OPCML; the disease is lymphoma.