In an effort to identify a possible association among epigenetic changes, urinary bladder cancer prognosis and early-recurrence, we analyzed the methylation pattern of CDH1, RARB, SFN and RASSF1A genes in 54 fresh samples of urinary bladder cancer, 14 of which were paired with tumor-adjacent normal urothelium; 39 paraffin-embedded UC primary tumor and/or recurrence matched with 23 bladder washing sediments obtained from 20 patients under post-surgical monitoring. This evidence concerns the gene RASSF1 and neoplasm.