BRCA1 and breast carcinoma: For example, the breast cancer cell line cohort included two splice site mutants that escaped detection by PAC because each had a second transcript length of major intensity that resulted from cryptic splicing (BRCA1 c.5396+1G>A in MDA-MB-436 [14] and p16 c.150+2T>C in MDA-MB-436 (Nagel et al., submitted for publication).