In the same group of patients, TFF3 assay was able to detect 60% (6 out of 10) of EEC at stage I. When the CA125 cutoff level was decreased at 20 U ml−1, as shown in Table 3, its sensitivity when compared with TFF3 remained significantly lower (i.e., 32 vs 56%, respectively). This evidence concerns the gene TFF3 and exstrophy-epispadias complex.