These additional mutations are likely to occur in molecules involved in the same biological pathways as RUNX1, as hemizygous loss of several molecules in the same biological pathway (e.g. RUNX1 and SPI1) is thought to be almost as tumorigenic as homozygous loss of one molecule (e.g. homozygous RUNX1 mutation in AML-M0) [15]. This evidence concerns the gene RUNX1 and acute myeloid leukemia with minimal differentiation.