Next the diagnostic stained tissue sections of surgical specimen demonstrated that it was, in fact, a lobular carcinoma of the breast (Figures 3a, b, c), with this immunoassaying profile ER + (figure 3d) (60%); PR + (40%); Ki67: 5%; human epidermal growth factor receptor 2 (Her-2) (DAKO) negative. This evidence concerns the gene ESR1 and breast lobular carcinoma.