In our series of 44 primary MSI CRCs, we further determined the relative in vivo expression of 18 MSI target genes by real-time quantitative RT-PCR using experimental conditions that we previously determined in a series of CRC cell lines (ATR, BAX, BLM, CBF2, CDX2, GRB14, GRK4, IGF2R, MBD4, MSH3, MSH6, RAD50, RBBP8, RECQL, RIZ, TCF4, TFDP2, TGFBR2) (Figure 3 and also Table S3 for the mutational status of these 18 genes in MSI CRCs) [14]. The gene discussed is GRK4; the disease is colorectal carcinoma.