TP53 and neoplasm: Brennan et al, (1995) first reported the use of TP53 mutation for the detection of the residual tumour cells in surgical resection margins to predict locoregional recurrences. Various groups have also investigated surgical margins with other molecular markers such as eIF4E, PSA, tyrosinase and microsatellite instability to predict locoregional recurrences (Proebstle et al, 1996; Theodorescu et al, 1999; Sardi et al, 2000; Nathan et al, 2002; Temam et al, 2004).