While cytokine mix tended to decrease Toll-like receptorsgene expression in all groups studied,LPS had more variable effects: enhancement of TLR2 receptors was observed in healthycontrols while significant depression of TLR4 expression wasnoticed in patients with osteoarthritis.These observations confirm that TLRagonist similarly to endogenous cytokines may modulateexpression of Toll-like receptors onleukocytes and suggest that thismodulation may vary in cells derived from patients with different form of rheumatic pathology or from healthy persons. The gene discussed is TLR4; the disease is osteoarthritis.