Importantly, our immunohistochemical approach showed a clear and almost exclusive FZD3/6/7-staining in HCC cells in tumours and hepatocytes in peritumours, demonstrating that the FZD3/6/7-mediated signalling could be specifically carried out by cancerous cells within HCC tissues, but not by mesenchymal or epithelial biliary cells. This evidence concerns the gene FZD3 and neoplasm.