However, when we analyzed TLE6 mRNAs bioinformatically, we identified a previously identified alternative spliced isoform of TLE6 (TLE6D) (Genbank Accession #BX375733) that contains only the C-terminal WD repeat domain of TLE6, and therefore has the same domain structure as mouse Tle6-like (Figure 4B) To understand expression of TLE6A (full-length isoform) and TLE6D in human CRC, we generated three sets of RT-PCR primers: one for the TLE6D N-terminus, one crossing the splice junction that is specific for TLE6D and one that detects TLE6A but not TLE6D (Figure 4B). The gene discussed is TLE6; the disease is colorectal carcinoma.