In the present study, changes in the number of neutrophils that expressed CXCR3, IFN-γ, CXCL9, and CXCL11 in the MLN, IL-12p40 and CXCL10 in PP as well as IFN-γ and TNF-α in LP of mice during Mycobacteria-enhanced colitis suggest that neutrophil subsets are differentially compartmentalized and regulated to propagate colitis. The gene discussed is CXCR3; the disease is colitis.