SNP rs6495446, an intronic SNP in the gene MTHFS on chromosome 15q25.1, was significantly associated with CKD in white ARIC participants at study visit 4 (OR 1.24 per each C allele, 95% confidence interval 1.09–1.41, p = 0.001), replicating the trait and risk allele observed in the initial screen. The gene discussed is MTHFS; the disease is chronic kidney disease.