In the present study, we further found that melittin and bee venom significantly reduced inflammatory stimuli (LPS and SNP)-induced activation of JNK signal, and the JNK signal specific inhibitor SP600215 suppressed the inhibitory effect of melittin and bee venom on the NF-κB activation, and inflammatory reaction in Raw 264.7 macrophages and synoviocytes obtained from rheumatoid arthritis patients. The gene discussed is NFKB1; the disease is rheumatoid arthritis.