ACAN and rheumatoid arthritis: However, identical bands of 10, 25 and 45 kDa were observed in both healthy controls, as well as RA patients after staining with F-78 (Figure 5a), different from the G1/G2 ELISA results (Figure 4), indicating that these fragments are a part of normal aggrecan turnover, and therefore, do not reflect a pathological turnover-process.