Cells were isolated by automated CD146 driven magnetic isolation, and assay accuracy, sensitivity, linearity and precision were assessed, Hereafter, nucleated (DAPI+) cells, expressing CD105 but lacking CD45 were enumerated in 249 healthy donors and 206 patients with metastatic cancer (Rowand et al, 2007). This evidence concerns the gene PTPRC and metastatic malignant neoplasm.