Cells were isolated by automated CD146 driven magnetic isolation, and assay accuracy, sensitivity, linearity and precision were assessed, Hereafter, nucleated (DAPI+) cells, expressing CD105 but lacking CD45 were enumerated in 249 healthy donors and 206 patients with metastatic cancer (Rowand et al, 2007). Here, MCAM is linked to metastatic malignant neoplasm.