To verify this hypothesis, we first analyzed the expression of the miRNA 17-5p-92 cluster in five neuroblastoma cell lines expressing MYCN at either low level (SH-EP and SK-N-AS) or high level due to MYCN overexpression (SH-SY-5Y) or amplification (LAN-5 and IMR32) (Figure 1A). This evidence concerns the gene MYCN and neuroblastoma.