TWIST1 and neoplasm: Based on this finding, we conducted further analysis of the methylation status of RASSF1A, CCND2, GSTP1, TWIST, and APC. Second, we analyzed the differences in the methylation status of these five tumor-related genes between premenopausal and postmenopausal, T1 and T2/T3, and LN metastasis negative (N0) and positive (N1/N2) subgroups using univariate analysis.