RUNX3 and pancreatic neoplasm: We examined LOH at this gene locus in 1p36 with microdissected DNA, the DNA-methylation status by methylation-specific polymerase chain reaction (MSP) and sequencing, and the mutation of RUNX3 by reverse transcription-polymerase chain reaction (RT-PCR) single-strand conformation polymorphism (RT-PCR-SSCP) in 32 primary pancreatic cancer tissues and corresponding noncancerous tissues.