We confirmed glioma-specific splicing for eight genes represented by 21 RefSeq entries: A2BP1, BCAS1, CACNA1G, CALD1, CLTA, KCNC2, SNCB and TPD52L2. The most dramatic cassette exon changes occurred in regions where the DE values approached 0 and the AS scores were above 13 as was the case with both CLTA and TPD52L2. The lack of glioma-specific splicing was confirmed for 12 genes (26 RefSeq entries) with p-value > 0.05: ALG12, CASP2,EMID1, FGFR1, PCNT2, LAIR1, MDM2, MNT, NAV2, PACSIN1, PECAM1 and TPM1. Overall, RT-PCR results were concordant with array data for 47 of 78 RefSeq entries (60%). Here, PACSIN1 is linked to central nervous system cancer.