JH Lagendijk et al [10] have also observed in their study that although the immunostaining patterns show a considerable overlap, the breast carcinomas were typically positive for GCDFP-15 and often for ER, and negative for vimentin whereas colonic carcinomas showed prominent positivity for CEA and CK20, while no staining was seen for ER and vimentin. Here, ESR1 is linked to breast carcinoma.