In this study, we constructed a PDGFR-β short hairpin RNA (shRNA) expression vector, which showed high gene-silencing efficacy and an antiproliferative effect in vitro, and investigated its antifibrogenic effect on experimental hepatic fibrosis induced by dimethylnitrosamine (DMN) and bile duct ligation (BDL). The gene discussed is PDGFRB; the disease is Hepatic fibrosis.