To test whether differential protein binding could alter the ability of the susceptibility alleles to activate transcription, we multimerised oligonucleotides overlapping both the Oct-1/Runx2 and the C/EBPβ binding sites, cloned these in both orientations upstream of the luciferase reporter gene in pGL3Enh (Figure 4A), and assayed them in three breast cancer cell lines (PMC42, HCC70, and T47D). Here, RUNX2 is linked to breast cancer.