In a panel of 20 colon cancer cases, all these genes except IRX5, TFAP2E and TFAP2C showed significantly higher methylation in cancer by t-test analysis (P<0.001 for NKX2-5, DPYS SPOCK2, GALR2 and SLC16A12; P = 0.008 for FOXN4) (Figure 3C, 4B and Table S3). The gene discussed is NKX2-5; the disease is malignant colon neoplasm.