To test the efficacy of loading DCs with tumor antigen by RNA electroporation we used TC-1 cells, a mouse adenocarcinoma cell line generated by cotransfection of lung epithelial cells with HPV-16 E6 and E7 genes and H-Ras [31], which has been used to test E6 and E7-targeted tumor immunotherapy [32-34]. Here, HRAS is linked to neoplasm.