Western blot analysis of intact male mouse bladders indicated that wild-type animals expressed more of the ~ 50 kDa TSP1 fragment (previously shown to have a neovascularization inhibitory role in vivo) [26] than their tumor-bearing UPII-SV40T transgenic counterparts, supporting an anti-angiogenic role for TSP1 in the exophytic growth of UPII-SV40T BC. This evidence concerns the gene UPK2 and breast cancer.