To verify that the transient expression of the promoter–luciferase constructs were authentically regulated when integrated into the Toxoplasma genome, we have developed several transgenic lines from the Type II-Prugniaud strain (cyst forming) that possesses an integrated copy of the firefly luciferase coding region under control of the BAG1 promoter (results for clone IC2 is shown in Fig. 3B). The gene discussed is BAG1; the disease is cyst.