The final analysis between the original and transplanted tumors showed that several of the upregulated genes in the transplanted tumors were associated with growth, metastasis, and cancer stem cells, such as Wnt inhibitory factor 1 (Wif1), Notch gene homolog 4 (Notch4), Hormonally-regulated Neu-associated kinase (Hunk), Sprouty homolog 1 (Spry1), Twist gene homolog 1 (Twist1), and Aldehyde dehydrogenase 3 family, member B1 (Aldh3b1). This evidence concerns the gene TWIST1 and cancer.